Laboratory for Optical Diagnosis and Imaging

Department of Biomedical Engineering

Texas A&M University

Director: Javier A. Jo, Ph.D. (


The Laboratory for Optical Diagnosis and Imaging was established within the Department of Biomedical Engineering at Texas A&M University in 2007. The overall mission of our laboratory is to develop technologies that could aid to the understanding of the underlying physiology and biology in various pathological conditions, and to translate such technologies into the clinical arena.

The focus of the laboratory is to develop optical spectroscopy and imaging technologies and related signal and image processing tools for quantifying nondestructively the structure, molecular composition, and physiological state of biological tissues with both macroscopic and microscopic resolutions. Early tissue pathological transformation are accompanied by subtle changes in tissue microstructure, biochemical composition and physiological regulations. Thus, our hope is that some of our developing technologies will help to clinically detect diseases during their early stages as well as to guide, monitor and personalize clinical interventions.

Multimodal Optical Imaging for Cancer Diagnosis. We are integrating complementary optical imaging technologies as tools for in-vivo non-destructive early detection of epithelial cancer. The image above shows a high-resolution 3D image of the oral mucosa of a hamster cheek pouch obtained with Optical Coherence Tomography (OCT), superimposed by a 2D superficial biochemical map obtained with Fluorescence Lifetime Imaging (FLIM). The white arrow shows a small tumor having distinct morphology and biochemical makeup.

Recent Selected Publications (Click title for PDF)

  1. 1.S. Shrestha, M.J. Serafino, J. Rico-Jimenez, J. Park, X. Chen, S. Zhaorigetu, B.L. Walton, J.A. Jo, B.E. Applegate. Multimodal optical coherence tomography and fluorescence lifetime imaging with interleaved excitation sources for simultaneous endogenous and exogenous fluorescence. Biomedical Optics Express, 7(9): 3184-3197, 2016.

  2. 2.P. Pande, S. Shrestha, J. Park, I. Gimenez-Conti, J. Brandon, B.E. Applegate, J.A. Jo. Automated analysis of multimodal fluorescence lifetime imaging and optical coherence tomography data for the diagnosis of oral cancer in the hamster cheek pouch model. Biomedical Optics Express, 7(5): 2000-2015, 2016.

  3. 3.B.H. Malik, J.M. Jabbour, S. Cheng, R. Cuenca, Y.S. Cheng, J. Wright, J.A. Jo, K.C. Maitland. A novel multimodal optical imaging system for early detection of oral cancer. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology; 121(3): 290-300, 2016. doi:10.1016/j.oooo.2015.10.020

  4. 4.Z. Meng, G.I. Petrov, S. Cheng, J.A. Jo, K.K. Lehmann, V.V. Yakovlev, M.O. Scully. Lightweight Raman Spectroscope Using Time-correlated Photon-counting Detection. PNSA 112(40): 12315-12320, 2015

  5. 5.D.U. Campos-Delgado, O. Gutierrez-Navarro, E.R. Arce-Santana, M.C. Skala, A.J. Walsh, J.A. Jo. Blind Deconvolution Estimation of Fluorescence Measurements Through Quadratic Programming. Journal of Biomedical Optics, 20(7): 75010, 2015.

  6. 6.J.A. Jo, J. Park, P. Pande, S. Shrestha, M.J. Serafino, J.J. Rico Jimenez, F. Clubb, B. Walton; L.M. Buja, J.E. Phipps, M.D. Feldman, J. Adame, B.E. Applegate. Simultaneous Morphologic and Biochemical Endogenous Optical Imaging of Atherosclerosis. European Heart Journal – Cardiovascular Imaging, 2015. (DOI:

  7. 7.P. Pande, S. Shrestha, J. Park, M.J. Serafino, I.B. Gimenez-Conti, J.L. Brandon, Y.S. Cheng, B.E. Applegate, J.A. Jo. Automated classification of optical coherence tomography images for the diagnosis of oral malignancy in the hamster cheek pouch. Journal of Biomedical Optics, 19(8): 086022, 2014.

  8. 8.S. Cheng, R. Cuenca, B. Liu, B.H. Malik, J. Jabbour, K. Maitland, J.A. Jo. Handheld multispectral fluorescence lifetime imaging system for in vivo applications. Biomedical Optics Express, Vol. 5, No. 3, 921-931, 2014.

  9. 9.O. Gutierrez-Navarro, D.U. Campos-Delgado, E.R. Arce-Santana, M.O. Mendez, J.A. Jo. Blind End-member and Abundance Extraction for Multi-spectral Fluorescence Lifetime Imaging Microscopy Data. IEEE Transactions on Information Technology in BioMedicine, , 18(2): 606-617, 2014.

  10. 10.J. M. Jabbour, S. Cheng, B. H. Malik, R. Cuenca, J. A. Jo, J. Wright, Y. S. L. Cheng, and K. C. Maitland. Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer. Journal of Biomedical Optics, 18(4): 046012, 2013.